The mortallty, amputation rates, and morbidity rates are high despite the successful removal of acute peripheral arterial occlusion. The principal cause of increased mortality and amputation rates is perfusion injury.
The importance of free-radical mediated damage during reperfusion after a period of is chemia has been documented in the kidney, heart, intestine, and brain. However, the role of oxygen free radicals in ischemia and reperfusion injury of the
skeletal
muscle has not been well defined partly because of the relative resistance of the tissue to normothermic ischemia.
In some clinical cases, skeletal muscle necrosis has been shown to occur much more severely after reperfusion, leading to high mortality and morbidity despite successful restoration of blood flow to the ischemic lower extremity.
This investigation aims to evaluate the role of free radicals and the role of taurine as well as radical scvengers in reperfusion injury of the skeletal muscle in male Wistar rats.
Various parameters, including blood levels of thiobarbituric acid(TBA) rectants, serum CPK, and the water content of calf muscles, were measured in the experimental setting, where infrarenal abdominal aorta was occluded for three hours followed
by
reperfusion with and without intraperitonial adminstration of radical scavenger (superoxide dismutase) and taurine (2-aminoethane sulfonic acid), although the exact mechanism of the injury remains unclear.
The rats were divided into four groups : group A : single laparatomy ; group B(ischemia-reperfusion group) : three-hour occlusion ; group C (SOD group) : three-hour occlusion with superoxide dismutase ; group D(taurine group) : three-hour
occlusion
with
taurine.
@ES The results were as follows :
@EN 1) The level of serum CPK and blood TBA increased steeply immediately after the abdominal aortic clamp was released in group B.
2) The increases in both serum CPK and blood levels of TBA reactants following reperfusion were diminished in group C and group D as compared with those in group B.
3) The water content of calf muscles showed a singnificant increase after reperfusion I group B, where as it did not change in group C and group D. Our results indicated that oxygen-derived free radicals may plays in important role in the
reperfusion
injury in the lower extromity and that taurine seems to play an important role in reducing lipid peroxidation.
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